Here we elaborate on the implementation details of FIMTrack and give an in-depth explanation of the used algorithms. However, so far there has not been an in-depth discussion of the technical aspects. This system in combination with the associated tracking software FIMTrack is already used by many groups all over the world. In the past we have introduced FIM, a novel imaging system feasible to extract high contrast images. elegans worms has become an integral subject of biological research. Imaging and analyzing the locomotion behavior of small animals such as Drosophila larvae or C.
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